Regulatory effect of compound Rhodiola on immune function in rats with high intensity training

Abstract Objective: To explore the mechanism of Chinese medicine to improve sports performance and reduce the decline of immunity caused by overtraining. METHODS: Fifty rats were randomly divided into 3 groups and subjected to corresponding treadmill training and testing. RESULTS: In the high-intensity training rats, the spleen cell antibody formation ability decreased, the white blood cell movement inhibition index increased, and the plasma ACTH and β-EP content was lower than that of the non-training control animals. The above indicators of the traditional Chinese medicine group taking compound Rhodiola while improving the intensity of the training showed an improvement trend. Conclusion: Compound Rhodiola can improve the exercise capacity and improve the immune function of rats with high-intensity exercise by affecting the neuroendocrine immune network.

　　 The regulation of Rhodiola Crenulata Compound on immune function of rats trained by heavy exercise

SHI Jian-Rong, ZHUANG Jian-Qing, GUO Rui-Xin et al. Shanghai University of Traditional Chinese Medicine, Shanghai 200032

　　 Abstract Objective: In order to study the effect of TCM on decreased immune function of the body after heavy exercise (HE) training. Methods: 50 rats were divided into three groups in the experiment. Results: A decline in the ability of antibody forming of Spleen cells and an increase of the inhibitor moving index of white cells were observed in the rats after heavy exercise training. simultaneous the contents of ACTH and β-EP in the plasma of the HE rats were lower than that of the normal control. group of TCM + HE, the rats were received Rhodiola crenulata Compound and forced heavy exercise, the above index were improved.This results indicated that this compound was effective in the exercise performance and could enhance immune function of the HE training rats Conclusion:. The Effects of the neuroendocrine-immune regulatory circuit were believed to be the possible mechanism for explaining the effect of this compound on promoting the immune function on the rats with heavy exercise trai Ning.

　　 Key words Heavy exercise training Immune function Neuroendocrine-immune Rhodiola Crenulata Compound

Moderate exercise can enhance the body's immunity, while excessive or intense exercise can reduce the body's immunity. For athletes, the usual intense training and intense competition are inevitable. Therefore, it is often found that some athletes lose their resistance after training and are prone to infection, which affects their athletic ability and even stops participating. Competition [ 1 ] . Therefore, how to reduce the decline of immunity and improve sports performance caused by overtraining has become a research hotspot. Traditional Chinese medicine provides a new way to solve the above problems. This study observed the changes of immune function in rat high-intensity training model and the effect of Chinese herbal compound Rhodiola on the immune function of rats in this model, and explored its possible mechanism.

　　 1 　 Materials and Methods

　　 1.1 Preparation of compound Rhodiola decoction The traditional Chinese medicines such as Rhodiola, Astragalus, Atractylodes, and Ophiopogon ( provided by Shanghai Pharmaceutical Co., Ltd. ) were prepared in proportion. After warming for 2 h, they were decocted and concentrated to 1.78 g of crude drug /ml. .

　　 1.2 Grouping and administration to an animal weighing about 50 190 g (3 months old) pure SD male rats (provided by Shanghai Experimental Animal Center of Medical) for adaptive treadmill training, excluding not run, would not run screened 37 rats were randomly divided into three groups: non-training control (Normal control) group 7 animals, the high intensity training (Heavy exercise, HE) group, 15 animals, medicine (TCM + HE) group, 15 Only animals. Medicines by oral administration, a dose of 10 ml / kg of body weight, 2 times a day orally. The non-training control group and the high-intensity training group were substituted with water for two times a day . According to the prediction experiment of dose-effect relationship, the optimal dosage of traditional Chinese medicine is equivalent to 25 times of the clinical adult dosage .

　　 1.3 Treadmill Training Method Non-training control animals are free to live in cages without applying exercise load. The high-intensity training group and the Chinese medicine group were trained with a domestic electric mouse running platform [ 2 ] , and trained for 6 days per week . 1 week at a speed of 15 m / min, the training day, 10 min; 2 weeks of running speed of 20 m / min, starting from 10 min daily daily increase at 60 min run; 3 weeks before the 3rd speed 30 m/min , time is 60 min , the running speed is unchanged after 3 days, the daily increase is 10 min , from 70 min/d to 90 min/d ; from the 4th week to the 8th week, both are 30 m/ The speed of min is trained for 90 min . During the training, 9 cases were eliminated due to animal foot injury or unexplained reluctance to run. By the end of the 8th week, there were 10 rats in the high-intensity training group and 11 rats in the Chinese medicine group .

　　 1.4 running performance test Day 1 Week 8, the high intensity training group and TCM group were fixed in the case where the running speed of 40 m / min to make every effort to rats can be continued as long distance running performance when the most.

　　 1.5 Sampling and test methods After the end of 8 w , the rats in the high-intensity training group and the Chinese medicine group were anesthetized with pentobarbital about 20 h after the last training stop, and blood samples and related tissue materials were sampled in the morning. Hemolytic plaque assay (PFC) for the determination of antibody-forming cells by chamber liquid phase method , and the leukocyte migration inhibition assay was determined by capillary method.

The motor inhibition index (IMI), plasma ACTH and β-EP were detected by the Shanghai Institute of Endocrinology and the Department of Neurobiology, Second Military Medical University.

　　 2 　 result

　　 2.1 Comparison of running scores between the high-intensity training group and the Chinese medicine group (TCM+HE) is shown in Table 1 .

　　 2.2 The white blood cell movement inhibition index, hemolytic plaque test and plasma ACTH and β-EP levels in the three groups were shown in Table 2 .

Table 1 Comparison of running scores between the high-intensity training group and the Chinese medicine group (min)

　　 Tab.1 The comparison of running time between the HE group and the TCM+HE group(min)

Note:t test , P < 0.05 , the mean:HE 189.03±95.20 , TCM+HE 254.48±103.25

Table 2 　 Leukocyte movement inhibition index, hemolytic plaque test and plasma ACTH and β-EP levels in three groups ( ±s)

　　 Tab.2 The results of IMI , PFC and the plasma contents of ACTH and β-EP in the three group animals ( ±s)

Note:F test , 1)compared with the normal control group P < 0.05 , 2)compared with the normal control group P > 0.05

　　 3 　 discuss

The body's immune cells have different transmitters and hormone receptors, which are regulated by the nervous and endocrine systems. In addition, immune cells secrete different transmitters, hormones and cytokines to achieve feedback regulation of the nervous, endocrine and immune systems themselves, forming neuroendocrine Immune network. Maintain normal immune function in the body under physiological activity. Its role is extremely complicated, such as growth hormone has a broad strengthening effect on immune function, while adrenal glucocorticoid has a wide range of inhibition, ACTH and β-EP show double regulation of immunity [ 3 ] .

The effect of exercise on immune function is related to exercise intensity and training time: the effect of long-term moderate or mild and moderate chronic exercise on the immune system may be the physiological response of the body to exercise stress. Acute intense exercise and long-term intense training promote the release of hormones with immunosuppressive effects in the body, inhibiting the body's immune function. After 5 w of intensive swimming training, the ratio of CD4 + /CD8 + cells decreased, T inhibited the production of cytokines, and the spleen cells decreased in response to exogenous IL-2 , and the secondary antibody response to TD antigen was affected. Inhibition, antibody production levels were significantly lower than the control group, and these results indicate that high-intensity training can cause immunosuppression [ 4 ] .

In this experiment, the rat treadmill exercise was used to simulate the repeated long-distance running of the periodic endurance item in the track and field exercise, resulting in a large-intensity exercise model in rats [ 2 , 5 ] . High Intensity Training is observed 8 weeks weighing animals (345.90 ± 23.75 g) was significantly lower than the non-trained control (392.29 ± 54.68 g, P < 0.05), the running test results are less than the duration of Medicines, PFC and IMI The experimental results showed that the humoral and cellular immunity levels of the animals in this group decreased, and the levels of ACTH and β-EP in plasma were lower than those in the non-training control group. Under normal circumstances, the body's plasma ACTH is measured immediately after a large amount of exercise, and the decrease in ACTH measured in this work may be related to the high-intensity training and sampling time (the experimental animals are sampled after 20 hours of the last exercise ) .

It is currently believed that ACTH and β-EP are important temperament regulating the endogenous regulation of the immune system, and the changes of the two are often accompanied by and are obviously related. Many authors believe that high-intensity exercise causes an increase in β-EP [ 6 ] , and there are also reports of a decrease in β-EP after exercise [ 7 , 8 ] . Vires believes that these different results are inconsistent with the training intensity, the amount of exercise the day before the experimental sampling, and the body's The rest is related. In addition, the observations of immune function regulation of ACTH and β-EP in vitro by different experimenters sometimes have opposite or dual effects, and the mechanism is not fully understood [ 9 ] .

Some of the important theories of TCM, such as the theory of righteousness, yin and yang, and the theory of dirty elephants, are similar to some basic concepts of immunology. Years of clinical practice has proved that the kidney-reinforcing method can improve the immune status of the body, and the functions of strengthening the spleen and tonifying the qi, supplementing the lung and replenishing qi and qi are all effective in improving the immune function of the body. The changes in immune function caused by traditional Chinese medicine are not only the result of the immune system, but also the total effect expressed by the neuroendocrine immune network [ 10 ] .

According to the literature reports and our findings: athletes with long-term and high-intensity training are mainly qi and yin. Therefore, this experiment uses anti-fatigue and hypoxia-resistant Rhodiola as the main drug, and supplemented with Astragalus, Atractylodes Rhizome, and Astragalus membranaceus High-intensity training of the body's function and improve exercise capacity. Among them, jaundice, which not only enhances immune function, but also has two-way regulation, can be considered as an immunomodulator. Astragalus has the effect of enhancing and regulating the body's immune function and improving the body's disease resistance. We have compared the effects of compound Rhodiola and unilateral. The experimental results show that the effect of compound on improving the motor ability of mice is more than unilateral, indicating that the compound strengthens the effect of nourishing qi and nourishing yin, and can better regulate the body function of long-term high-intensity training. The rats' exercise performance was improved after taking the high-intensity training; the cellular and humoral immune function and the plasma ACTH and β-EP levels were not significantly different from the normal control animals, indicating that there was a trend close to the normal group of animals, presumably related to the traditional Chinese medicine. It is related to the regulation of neuroendocrine dysfunction. About the author: Shi Jianrong, female, 35 years old, master's degree, associate researcher, mainly engaged in basic research of integrated Chinese and Western medicine;

Zhuang Jianqing, female, 47 years old, master's degree, associate researcher, mainly engaged in basic research of integrated Chinese and Western medicine

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