1. Remove the mouse scalp and clean the skull. Make a small hole in the selected position above the skull (see the Locator map) (using the precision high-speed drilling MF5360, the ring bit MF5176----this hole is used to place the probe base), this small hole needs to penetrate the skull. The ring drill is characterized by less cuttings.

2. Place 2-3 holes near the hole (using the drill bit MF5362) for mounting the screw MF5182. The purpose is to increase the adhesion of the probe base to the skull, so that the probe base does not fall off easily.

3. It is important to accurately select the location where the probe is embedded in order to get the most microdialysis samples. Using the stereo positioner MD3000, the probe base (MD2251 or MD2250) is buried in the hole above. Fix the probe base and screws with the adhesive MD1300.

4. Insert the probe (eg MD2200) into the probe base using the stereo positioner MD3000.

5. Select the flow rate of the propulsion controller (MD1000 or MD1020) according to the experimental needs. Insert the connecting flat wire above the propulsion controller into the jack of the micropropeller MD1001.

6. Fill the syringe (eg MDN0100) with the liquid required for the experiment and place it on the micropropeller. Only one syringe can be placed on top of this micropropeller. If you need to put 2-3 syringes on a micro-propeller, you can buy the syringe rack MD1002, and you can put 2-3 syringes on the MD1002.

7. Place the mouse with the probe embedded in the cage of the awake system MD1404. Put the collar MF5371 on the body of the mouse and hook the hook inside the awake system to the MF5371. Therefore, when the mouse moves, the hook also rotates. At the same time, the photoelectric sensor above sends a signal to the instrument that the mouse is moving, and the instrument will make The chassis of the awake system rotates. Of course, this rotation is opposite to the direction in which the mouse walks. The most important result is that the two tubes (infusion tube and dialysate tube) above the mouse body are not entangled or blocked. The liquid in the two tubes flows smoothly.

8. Connect the syringe----the probe with the tube MF5164 and the connector MD1510. The other interface of the probe is connected to the thermostated freeze collector MD1201 with a tube and a joint.

9. Check that the connections are correct.

10. Turn on the conscious system MD1404 power supply.

11. Place the collection tube inside the constant temperature refrigerated collector MD1201, turn on the constant temperature freezing collector power supply, and set the constant temperature freezing collector 5 parameters.

12. Turn on the power to the controller and the microdialysis instrument starts working.

Suggest

Microdialysis experiments differ from many experiments in that many aspects depend on the skill level of the operator.

1. The location of the probe should be accurate. The depth of the buried probe should also be accurate. The length of the membrane from which the probe is selected needs to be appropriate. The goal is to get the most microdialysis samples.

2. The probe needs to be careful, and it is necessary to avoid damage to the membrane of the probe, thereby prolonging the life and number of uses of the probe.

3. Processing before using the probe. And processing, rinsing, soaking at the time of use, the purpose is to extend the life and number of uses of the probe.

4. When connecting the syringe, probe, liquid selector switch, constant temperature freezing collector, etc., you need to be careful, connect the pipe, joint, and the above objects, when you insert/insert, on the one hand, you need to prevent leakage and blockage. On the other hand, it is also necessary to prevent damage to the connecting pipe and the joint.

5. Probes, connecting tubes, joints, consumables, careful, correct operation, can extend its service life and frequency.

6. Preparation of perfusion fluid, selection of flow rate. The parameters of the constant temperature freezing collector (delay time, collection time, etc.) are selected. Wait, you need to learn and summarize your own experience. In particular, embedding the probe is an important step in obtaining a large amount of microdialysis liquid, and the operator's technique is very important.

To make the experiment successful, the importance of the skill level of the operator is more important than the instrument itself. Excellent operators are easy to get micro-dialysis solution, the quantity is good, and the consumables (probe, connecting tube, joint) have long service life and many times.

Tip: When the micro propeller works, because the flow rate is very slow, only a few drops of liquid are introduced in a few minutes. The human eye can't see if the micro propeller is working, so sometimes the operator is psychologically worried that the micro propeller is not working. . There is a simple method: a small white spot (white tape or white sticky paper) is applied to the black cylinder at one end of the micro propeller. When the micro propeller is working, the black cylinder will rotate slowly, and the white point will Changing the position indicates that the micro propeller is working properly and dispels psychological concerns.