product description:
| Â English name | Â Human ACE (Angiotensin I Converting Enzyme) CLIA Kit | ||
|  Chinese name  |  Human angiotensin converting enzyme (ACE) chemiluminescence immunoassay kit  | ||
| Â Item number | Â E-CL-H0002c | Â species | Â Human / person |
| Â specification | Â 96T/Kit (8*12 strips) 48T/Kit (8*6 strips) | ||
|  Detection method  |  Double antibody sandwich method  | ||
| Â examination range | Â 0.781~50ng/mL | Â Sensitivity | Â 0.469ng/mL |
Fundamental
This kit uses a double antibody sandwich method. The anti-Human ACE antibody was coated on the plate, and the ACE in the specimen or standard was bound to the coated antibody during the experiment, and the free component was washed away. Biotinylated anti-Human ACE antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human ACE antibody binds to Human ACE bound to the coated antibody, and biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay, and a positive correlation was observed between the ACE concentration and the chemiluminescence value. The standard curve is used to determine the concentration of ACE in the specimen.
Steps
1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes.
2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes.
3. Wash 3 times
4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes.
5. Wash 5 times
6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C
7. Readings
8. Calculation of results
For more details, please visit
Animal medicine,Dexamethasone Sodium Phosphate,Pimobendan for Sale,Pimobendan Buy,Cloxacillin Benzathine Solubility,
China Veterinary raw ,Low Price Ampicillin supplier & manufacturer, offer low price, high quality Cas 7177-48-2 Ampicillin,Ampicillin Powder, etc.
This is Xi'an Evergreen Chemical Factory Co., Ltd.,We are professional supplier of raw Cosmetic Raw material and have run around 10years, just like Copper Peptide Ahk-cu Powder: Effectively promote the production of collagen and elastin, Skin Whitening powder ( sepi white powder, giga white powder and snow white powder), Anti-Wrinkle Products: Remove wrinkles and become younger and more radiant, Loss Weight Powder: Stay in good shape and live a healthier life and so on .And at same time , we are ISO Certificated Manufacture for 12 years, three Millions registered Capital, 320 Employees, Professional export experience to more than 60 Countries. Passed SGS third party certified. And nearly 10 years export experience to more than 60 Countries. Professional sales service with top quality,fast delivery, so if you are interested in these products , please contact us .we focus on the research, development, production and sales of Food Additives , Plant Extract ,Cosmetic raw powder , Nutrition Supplements and APIs Raw powder .We supply Medical and health services Hexapeptide-11 Active Ingredients, apha arbutin, hyaluronic acid, Professional and reliable Tranexamic acid, Ferulic aci, Professional export Kojic Acid Dipalmitate Powder, Giga white, snow white and sepi white etc.
Animal medicine,Dexamethasone Sodium Phosphate,Pimobendan for Sale,Pimobendan Buy,Cloxacillin Benzathine Solubility
Xi'an Henrikang Biotech Co.,Ltd , https://www.henrikangbio.com