Personal flow cytometry HPC-100 technology principle, technology innovation and application

Flow cytometry has always been expensive, expensive to maintain and use, and complicated to operate, which is a constraint to its ability to become a conventional instrument. In recent years, the emerging personal flow cytometer has gradually become the new darling of the laboratory due to its low price, simple operation, low maintenance and low cost. But the small flow instability is still one of its biggest problems.

In 2013, Handyem launched the revolutionary new generation personal flow cytometer HPC-100. Its core technology is the "F3FiberFlowFluidicsTM" flow chamber designed with fiber optic microfluidics, which can achieve sheath-free, ultra-small, anti-vibration performance. The emergence of the HPC-100 personal flow cytometer will completely change people's perception of traditional flow cytometry, and become a good helper to improve the efficiency of scientific research.

Four major technological innovations

1. Aviation-grade anti-shock performance Fiber-optic intervention flow chamber avoids the detection error caused by the reflection of the reflected and focused spot in the traditional flow cytometer flow chamber. It can be placed in the biological safety cabinet and the fume hood to prevent the sample from being corrected. Contamination, protection of operators, standard operation; can also be used for field inspection and offshore operations, and can be used at will.

2, the unique sample recovery without sheath fluid design section to avoid cell rupture caused by dilution of the sheath fluid, the sample can be recovered for microscopic observation or re-culture, saving and preserving your precious samples.

3, high-end streaming high data quality for the first time using 26-bit intelligent AD converter, beyond 20 million data accuracy, beyond the dynamic range of 7 orders of magnitude, can present more effective data.

4, ultra-compact design to achieve ultra-small volume is currently one of the most mainstream small flow cytometer volume, can be flexibly placed in the biosafety cabinet, easy to carry.

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1, non-sheath fluid design
The HPC-100's microfluidic design is currently an upgraded technology for non-sheathing power focusing. The fiber-optic, sheath-free flow cytometer reduces operating and maintenance costs and eliminates cumbersome laser spot correction for shock and portability. There is a great breakthrough.

2, high optical efficiency flow chamber built-in total reflection filter, side scattered light and fluorescence double collection and achieve secondary excitation, optical efficiency is 2.3 times more than ordinary flow cytometry.

3, all fiber introduction technology
The HPC-100 is the only flow cytometer on the market that uses fiber optics as a spatial filter to conduct laser light into the flow cell to excite samples. The excitation and emission fibers are integrated in the flow chamber to ensure that the laser can stably excite the cells in any environment. On the other hand, the fixed components ensure the vibration resistance of the flow chamber, so no correction is needed.

Application field
● Apoptosis/cells (Annexin, caspase, PI, mitochondrial membrane potential, cell surface death receptor, calcein)
● Cell cycle analysis and ploidy
● cell proliferation
● Intracellular NO and superoxide formation
● Multi-parameter immunophenotypic analysis
● Cell surface marker analysis (HLA-A, B, C)
● Pharmaceutical/Industrial/Plant Research & Quality Control
● Food/Beverage Detection & Quality Control
● Animal reproduction/health research
● Health diagnosis in poor and remote areas