Working principle and application of full-wavelength microplate reader

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The Microplate Reader is a professional instrument for reading and analyzing the results of enzyme-linked immunosorbent assay (EIA) experiments. The enzyme-linked immunoreactivity is carried out by an enzyme-catalyzed chromogenic substrate coupled to an antigen or an antibody. The result of the reaction is shown in color, and the antibody or antigen to be tested in the specimen can be judged by the depth of the color developed, that is, the value of the absorbance. concentration. The microplate reader is actually a disguised special photoelectric colorimeter or spectrophotometer. The basic working principle is basically the same as the main structure and photoelectric colorimeter.
First, the working principle of the microplate reader
The antigen or antibody is bound to the surface of a solid support and maintains its immunological activity. The antigen or antibody is linked to an enzyme to form an enzyme-labeled antigen or antibody, and the enzyme-labeled antigen or antibody retains both its immunological activity and the activity of the enzyme. The antigen-antibody complex formed on the solid phase carrier is separated from other substances by washing, and finally the amount of the enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the sample. After the substrate of the enzyme reaction is added, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the test substance in the sample, so that qualitative or quantitative analysis can be performed according to the depth of the color reaction. Due to the high catalytic frequency of the enzyme, the reaction effect can be greatly amplified, so that the measurement method achieves high sensitivity.


Second, the application of microplate reader
The microplate reader is widely used in clinical laboratory, biological research, agricultural science, food and environmental science. Especially in recent years, due to the application of a large number of enzyme-linked immunoassay kits, the microplate reader is in the field of reproductive health. The application is more and more extensive, and at the same time promotes the improvement of reproductive health technology. At present, in the domestic blood station clinical laboratory, the microplate reader has become an important tool for ELISA determination. The use of microplate readers to study phenol oxidase in agriculture provides a theoretical basis for the development of new pest control agents targeting this enzyme. Fumonisin is a recently discovered structurally related toxin metabolite produced primarily by several fungi that has been listed by the World Health Organization as one of several mycotoxins that were first studied in recent years. Therefore, the rapid and efficient detection of fumonisins is particularly important. Optical measurement of microplates using a microplate reader at 450 nm or 630 nm filters can detect fumonisins and has important applications in food safety. The growth curve generally refers to the total number of colonies CFU/mI. Or logarithm of the total number of colonies, log CFU/ml. A curve over time that can be used to demonstrate microbial growth and metabolic patterns. At present, there are shortcomings such as poor immediacy, cumbersome operation and easy pollution when measuring the suspension by spectrophotometer. Since the microplate reader is a fast, accurate and high-flux instrument, we always choose to use a microplate reader. The OD value of the bacterial suspension is measured to improve work efficiency and save time.
Thermofisher's new full-wavelength reader Multiskan GO supports endpoint, kinetic and spectral scanning measurements using xenon lamps as the source of light, wavelength selection from advanced grating systems, wavelengths from 200nm to 1000nm, and full spectrum in 1nm steps Scanning, including UV and NIR, is free to choose any wavelength. It has the dual function of microplate and cuvette measurement, and can be used as a full-wavelength microplate reader or as a full-wavelength spectrophotometer.


Multiskan GO can be operated independently by the built-in software of the instrument or by computer control using Skanlt software. Both softwares are intuitive, clear, flexible and easy to operate; and are available in eight languages ​​including Chinese.

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