1. The production of environmental strains promotes cleaner production. The bacteria room should be disinfected in advance. It is best to use 2 or more methods to cross-sterilize to prevent the generation of drug resistance of the bacteria. The bacteria bags that contain germs should be treated in a timely manner. Serious cases must be thrown away in time and buried in the production site.
2. Raw material selection is economical, practical, and has a wide range of raw materials. Do not use raw materials that have been mildewed or rotted. The proportion of various substances in the culture medium should be properly formulated, and in particular, a suitable ratio of carbon to nitrogen should be used. The appropriate PH value should be selected for different kinds of edible fungi. The moisture content of the culture material should be appropriate, the seasoning should be uniform, and the good material should be packed in time.
3, sterilization and sterilization is the key to the production of bacteria and God, sterilization must be thorough. Atmospheric pressure steam sterilization Note that there is no dead space in the sterilization chamber; high-pressure steam sterilization should thoroughly exhaust the cold air to prevent false pressure and affect the sterilization effect.
4, inoculation to select good strains. Inoculation must be performed under aseptic conditions. The culture medium, inoculation tool, and operating environment should be strictly sterilized. The inoculation process should strictly adhere to the aseptic procedure and must not be mixed with any bacteria to ensure the quality of the strain.
5, culture strains during the culture process should always check and found that there is a bacterial infection should be immediately removed, there is no timely germination of the bottle (or test tube) should be individually placed or replanted. Frequently check and adjust the temperature so that the mycelium grows at a moderate temperature. When the mycelium is about to grow, reduce the culture temperature by 2-3°C, then the hyphae will be more robust and strong, with proper ventilation and keeping the air in the bacteria room fresh.
6. The storage bacteria should be promptly used after being grown well. At this time, the hyphae are in the best growth period, and inoculation into new culture materials can show strong adaptability. Storage is too long, cultivation time is too long, nutrient consumption is more, mycelium is aging, vitality is significantly reduced, and later pollution is increased. If you do not use it temporarily, store it in a cool, dry, clean room away from the light.
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