Experts in the prevention and control of pests and diseases said that after the fall of autumn, corn is vulnerable to drill-type pests such as armyworms and corn borers. These pests will directly damage the corncob, which will cause the corn to die slowly. The height of corn in this period has already been almost one person high, so it is more troublesome to fight drugs by hand. If you use a drugs fight, you can achieve twice the result with half the effort.
Preventing the lodging of corn, if it falls, has a very large impact on its production. There are several main preventive measures. The first is to cultivate the soil and increase the number of supporting roots, so that the lodging resistance can be improved. Secondly, the potassium dihydrogen phosphate is applied to the page based on the application of potassium fertilizer. The toughness of the plants is greatly enhanced. At this time, if it is really encountering storms and other attacks that are difficult to confront, if there is a lodging, it is necessary to take active remedial measures, such as artificially raising the soil or cultivating the soil to restore itself.
Artificial pollination continues in high temperature days in summer, which affects the pollination of corn. This results in a low rate of corn cob seeding, so artificial pollination is necessary for this situation.
Corn in the drought-preventing and sorghum-grain period has a high demand for water. If water is scarce, it will affect the formation of the grain or the fruit is not full, and the yield will naturally decrease. Therefore, when the dry weather continues to occur, it is necessary to fill the water in time.
If the appropriate late harvest does not affect the second planting, the corn harvest period can be appropriately postponed, which will increase the grain yield of the corn kernels and increase the yield. So when the corn glutinous leaves become dry and loose, the grain begins to harden and appears golden yellow. When the grain milk line is completely invisible, the corn is in the ripening period, and the harvest is the highest. At the same time, the appropriate late harvest of corn is high in quality and the protein and amino acid content will increase.
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MLPA ( Multiplex Ligation-dependent Probe Amplification) method detects multiple copy number changes of genes or loci sites. Nowadays, MLPA is used to check large numbers of hereditary disorders and tumour profiling.Since Dutch Scientist Jan Schouten first invented it, the MLPA method was first published in 2002 'Nucleic Acid Research'. The principle of the MLPA is to apply the specific probe design targeting a region of interest on each sample DNA. MLPA method consists of the following steps:
Denaturation > Hybridization Ligation > PCR MLPA Amplificons Capillary Electrophoresis >Data Analysis
PCR reaction consists of the three steps: denaturation, primer annealing and primer elongation. Those steps of PCR amplification are repeated many times. The fluorescence-labeled primers, which will go through the capillary electrophoresis to pass a detector, are incorporated into the size of the amplification products. The measured fluorescence was visualized as a peak pattern, the so-called electropherogram. The raw data from the capillary electrophoresis analyzer forms the input of the MLPA analysis.
The genetic analyzers of Superyears Gene can be used not only for Sanger sequencing but also for fragment analysis. Fragment analysis First, it obtains the fluorescence-labelled DNA fragments, performs the capillary electrophoresis using a Genetic Analyzer and compares the sample's relative size standard with the designed size standard markers through the analysis software.
The genetic analyzer of Superyears Gene can be used in the capillary electrophoresis during the process of the MLPA experiment, and the data obtained can be used for (compatible) professional analysis software.
Genetic Analyzer For Mlpa,3130Xl Genetic Analyzer,Genetic Analyzer Capillary Electrophoresis,Genetic Analyzer Gene Sequencing Mlpa
Nanjing Superyears Gene Technology Co., Ltd. , https://www.superyearsglobal.com