On December 16, 1999, experts from the Provincial Department of Ocean and Fisheries organized a technical appraisal of the "Zionia urchin artificial breeding project" undertaken by the Daya Bay Fisheries Experimental Center in our province. The results showed that the technical level reached the domestic leading level.
Purple sea urchin is mainly distributed in the southeast coast of China. Living in the rocky sea area within 70 meters depth; breeding season from April to November, breeding peak period from April to June; growth suitable salinity 25-30 ‰; suitable water temperature 15-30 C. The main economic value of sea urchin is its gonad processed into fresh sea urchin yellow. Fresh sea urchin yellow has a high nutritional value, its protein content exceeds water, rich in lecithin and vitamin A, B1, B2, B6, B12, C, E and free amino acids. Products exported to Japan and other places, about 400,000 yuan per ton, is one of the higher export value of seafood. Our province now has an annual output of about 150 tons of sea urchin yellow, and sea urchin production mainly depends on natural fishing, and its quantity is limited. The successful research of the purple sea urchin artificial nursery project provided the seedling and seed protection for the province to carry out the planned and planned propagation of sea urchins in the future.
Induction of spawning: Take mature individuals, inject 36 g/L of potassium chloride solution 3-5 ml from the membrane, and place the progeny urchin bladder in a 500-ml egg-collecting beaker with the urchins facing downwards. The males and females were collected after collecting eggs.
Insemination and Washing Eggs: Use a semen that has been confirmed by microscopy to be of good quality. Add 1 drop of 3 drops of concentrated semen from different males to the roasting ovum and insemination with a glass rod. Let stand for 20 minutes and then put it in a 20 litre sink. Then wash eggs 3 times within 1 hour. Wash the eggs with water change.
Split-chamber incubation: Inoculate with 20 liters of transparent square sinks. Incubate 10 liters of water. Collect 1 million fertilized eggs per well. Let stand until the next morning. Then observe each hatching tank in a microscope. The juvenile's pros and cons were selected and put into a one-ton transparent plastic sea urchin hatching tank for development and management of micro-inflated larvae. Cultivation density is 0.8-1.0/ml.
Cultivate the environment: Water temperature above 25°C, salinity above 25°C; pH above 7.5. From the beginning of larval rearing until the 6th wrist period, 100% of the water is changed every day. 60% of the water is changed every day after the wrist period, and the water is sieved with a screen size of 80 μm and 100 μm. Micro-inflated cultivation.
Feeding and suction: single-horned algae for bait. The daily feeding amount of the four wrists was fed only once in the morning, and the six wrists were fed once in the morning and evening. The bottom of the pool is contaminated once a day.
Larval development: At a water temperature of 28°C and a salinity of 28°C, division begins about 45 minutes after fertilization; after about 5 hours it develops into the blastocyst stage; and about 7 hours after the ruptured membrane develops into a floating blastocyst larvae. After the first development of the larvae, four-armed larvae, six-armed larvae, and eight-armed larvae, most of the eight-armed larvae later expanded from the vestibular compound in the late 10 days. After the days of metamorphosis to sea bladder.
Collecting seedlings artificially: In the late eight-wrist period, when more than 30% of larvae have multiple tube feet protruding from the vestibule complex and a thorny forceps appears, picking is performed. During the development of planktonic larvae of purple sea urchins, the emergence of eight-vessel forceps in the late stage is the key to decision-making. The ratchet forceps is a method for the preservation and preservation of aquatic products of sea urchin juveniles.
Purple sea urchin is mainly distributed in the southeast coast of China. Living in the rocky sea area within 70 meters depth; breeding season from April to November, breeding peak period from April to June; growth suitable salinity 25-30 ‰; suitable water temperature 15-30 C. The main economic value of sea urchin is its gonad processed into fresh sea urchin yellow. Fresh sea urchin yellow has a high nutritional value, its protein content exceeds water, rich in lecithin and vitamin A, B1, B2, B6, B12, C, E and free amino acids. Products exported to Japan and other places, about 400,000 yuan per ton, is one of the higher export value of seafood. Our province now has an annual output of about 150 tons of sea urchin yellow, and sea urchin production mainly depends on natural fishing, and its quantity is limited. The successful research of the purple sea urchin artificial nursery project provided the seedling and seed protection for the province to carry out the planned and planned propagation of sea urchins in the future.
Induction of spawning: Take mature individuals, inject 36 g/L of potassium chloride solution 3-5 ml from the membrane, and place the progeny urchin bladder in a 500-ml egg-collecting beaker with the urchins facing downwards. The males and females were collected after collecting eggs.
Insemination and Washing Eggs: Use a semen that has been confirmed by microscopy to be of good quality. Add 1 drop of 3 drops of concentrated semen from different males to the roasting ovum and insemination with a glass rod. Let stand for 20 minutes and then put it in a 20 litre sink. Then wash eggs 3 times within 1 hour. Wash the eggs with water change.
Split-chamber incubation: Inoculate with 20 liters of transparent square sinks. Incubate 10 liters of water. Collect 1 million fertilized eggs per well. Let stand until the next morning. Then observe each hatching tank in a microscope. The juvenile's pros and cons were selected and put into a one-ton transparent plastic sea urchin hatching tank for development and management of micro-inflated larvae. Cultivation density is 0.8-1.0/ml.
Cultivate the environment: Water temperature above 25°C, salinity above 25°C; pH above 7.5. From the beginning of larval rearing until the 6th wrist period, 100% of the water is changed every day. 60% of the water is changed every day after the wrist period, and the water is sieved with a screen size of 80 μm and 100 μm. Micro-inflated cultivation.
Feeding and suction: single-horned algae for bait. The daily feeding amount of the four wrists was fed only once in the morning, and the six wrists were fed once in the morning and evening. The bottom of the pool is contaminated once a day.
Larval development: At a water temperature of 28°C and a salinity of 28°C, division begins about 45 minutes after fertilization; after about 5 hours it develops into the blastocyst stage; and about 7 hours after the ruptured membrane develops into a floating blastocyst larvae. After the first development of the larvae, four-armed larvae, six-armed larvae, and eight-armed larvae, most of the eight-armed larvae later expanded from the vestibular compound in the late 10 days. After the days of metamorphosis to sea bladder.
Collecting seedlings artificially: In the late eight-wrist period, when more than 30% of larvae have multiple tube feet protruding from the vestibule complex and a thorny forceps appears, picking is performed. During the development of planktonic larvae of purple sea urchins, the emergence of eight-vessel forceps in the late stage is the key to decision-making. The ratchet forceps is a method for the preservation and preservation of aquatic products of sea urchin juveniles.