Human parvovirus infection can cause a variety of diseases with different clinical symptoms such as infectious erythema, vascular purpura, joint disease, finger and toe tingling. The diseases caused by the virus are mostly self-limiting, and the prognosis is mostly good, but if the patient's immune function is low, it can lead to prolonged disease and chronic menstruation.
[Etiology] Human parvovirus (HPV) is a small DNA virus known as Zui, belonging to the parvovirus genus of the Parvoviridae. It was first discovered in the peripheral blood of blood donors in 1975 and was named human. Microvirus B1. . The virus particle is round, has a bare nucleocapsid, contains a single strand of DNA, its size is 545kD, has autonomous replication ability, the virus can not be tissue culture, can only grow in the young red blood cell nucleus of human bone marrow cells . B: Bone marrow culture. It was demonstrated that there are 9 mRNAs, 5 of which express proteins; 2 encode 84kD structural proteins, 2 encode 58kD structural proteins, and 1 encodes 77kD non-structural proteins. Structural proteins form the nucleocapsid, and the function of non-structural proteins is unclear.
[laboratory diagnosis]
1. Specificity check (1) Virus particle detection.
(2) Serological testing.
(3) Genetic diagnosis.
2. Non-specific examination of histomorphometric examination.
[Result analysis and judgment]
1. Detection of virus particles Direct observation of viral particles in serum by electron microscopy can be confirmed by positive.
2. Serological tests can be performed by radioimmunoassay or enzyme-linked immunosorbent assay for viral antigens or characteristic IgM antibodies or high titer IgG antibodies, all of which contribute to the diagnosis of this viral infection.
3. Genetic diagnosis The viral DNA is detected from the blood of a patient by dot blot hybridization or PCR.
4. Histomorphometric examination Intranuclear inclusion bodies can be found by observing infected mature red blood cells under a light microscope. Most of the inclusion bodies are shallow in the center, and the surrounding chromatin ring-like edge phenomenon is similar to the lantern, which can be suspected to be infected with the virus.
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