mCherry is a red fluorescent dye widely used as a tracer in biotechnology, including molecular markers and localization of cellular components. Unlike other GFP proteins and other green fluorescent protein variants isolated from Victoria jellyfish, mCherry and most other red fluorescent proteins are proteins isolated from the Discosoma species. mCherry is superior to other fluorescent protein tags because of its color and light stability of monomer molecules, its maximum excitation and emission 
Do not be 587nm and 610nm.
How to choose the right mCherry tag antibody?
When choosing mCherry antibodies, we mainly rely on our own needs, especially the needs of experimental applications. Among the factors to be considered are the type of application detection of mCherry antibody, the clonality of mCherry antibody, and the source of preparation. For example, can the mCherry antibody be used for immunofluorescence detection? Can it be used for immunoprecipitation IP experiments? Do I need a monoclonal GST-tagged antibody with better specificity? Do I need to consider whether to choose the source of the mouse or the source of the rabbit?
In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl mCherry antibody (if the dilution ratio of WB assay is 1:4000, the final use solution is equivalent to 400 ml), 1 ml of mCherry antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml).
I bought Abbkine's mCherry tag antibody for WB and IF experiments, both of which were ideal; I tried the IP experiment later, although the instructions were not written, but the effect exceeded expectations, very good. ——One customer of Wuhan Tongji Hospital
| Product name and description | Brand | Item number | product manual |
| Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | Abbkine | A02080 | Details |
Why recommend the Abbkine brand mCherry antibody?
There are many imported brands of mCherry antibody, including Sigma, Novagen, Qiagen, Abcam, etc. Among them, Abcam has a star-recommended mCherry antibody named Anti-mCherry antibody [1C51] (item: ab125096), which is Abcam The only recommended mCherry tag antibody is available for IF/ICC, IHC and WB experiments. The antibody is a monoclonal antibody, the clone number is 1C51, and the 2013 price of this antibody in China is ¥3996/100 μl. Abcam's antibodies are highly recommended in terms of brand awareness, quality and after-sales, and of course the price is higher.
In comparison, we recommend Abbkine's mCherry tag antibody, Anti-mCherry Tag Monoclonal Antibody (9D3) (Cat. No. A02080). Like Abcam, the immunogen of the AChkine mCherry antibody is an mCherry recombinant protein that recognizes the mCherry fusion protein that recognizes the mCherry protein and overexpresses in cells. However, its price is 60% lower than Abcam. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. The antibody can be used in a variety of different applications such as Western Bloting, immunocytochemistry and immunofluorescence ICC/IF, and has been reported by customers to be used in immunoprecipitation IP experiments.
Some domestic suppliers can also provide sub-packaged mCherry antibodies, but there are deficiencies in brand and quality assurance; in general, their potency is not very high. There are also some restrictions on brand after-sales. Abbkine's mCherry tag antibody (Cat. #A02080) In the conventional WB experiment, the recommended initial dilution ratio is 1:1000. Actually in Abbkine application, there is a dilution ratio of 1:5,000 or higher, depending on the customer. Specific experimental conditions are optimized.
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| Product name and description | Brand | Item number | product manual |
| Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | Abbkine | A02080 | Details |
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Abbkine also offers large packages of 1ml and larger custom-made mCherry tag antibodies, making it ideal for routine testing of mCherry immunology. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.
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