EB dye solution manual number: E1020
Specification: 5ml (10mg/ml)
Storage: Store at room temperature and protect from light, valid for at least 1 year.
product manual:
Ethidium bromide is a highly sensitive fluorescent stain commonly used for nucleic acid staining after agarose gel and polyacrylamide gel electrophoresis. The ethidium bromide is combined with DNA and emits orange-red fluorescence under excitation by an ultraviolet light transmissive device, which can be photographed using a Polaroid negative film or a gel imaging processing system with a CCD imaging head. The fluorescence yield of the ethidium bromide-DNA complex is 20-30 times higher than that of the dye without DNA binding, so when the gel contains free ethidium bromide (0.5 ug/ml), it can be detected as little as 10 ng of DNA bands.
Usage: 1, Glue: 100ml agarose gel solution (concentration is generally 0.8%~2%) is melted in a microwave oven, cooled to about 60 °C (not hot), then add 5-10ul EB nucleic acid dye, Gently shake and pour the gel (to avoid air bubbles). After the gel is completely solidified, apply the electrophoresis. After electrophoresis, observe the photo under the violet light. (Note: due to the presence of the dye, the electrophoretic mobility of the linear DNA is about Reduce by 15%).
2. Bubbling: After agarose gel electrophoresis, the gel was immersed in running buffer containing EB (0.5 ug/ml) or deionized water, and stained at room temperature for 15-45 min (depending on gel thickness). Decolorization with deionized water or 1 mM MgSO4 solution for about 10-30 min at room temperature can reduce background fluorescence (optional).
Precautions:
1, this product is a strong mutagen, please pay attention to safety when using.
2, For your safety and health, please wear a lab coat and wear disposable gloves.
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G8140 GoldView Type I Nucleic Acid Stain (10000×)
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Specification: 5ml (10mg/ml)
Storage: Store at room temperature and protect from light, valid for at least 1 year.
product manual:
Ethidium bromide is a highly sensitive fluorescent stain commonly used for nucleic acid staining after agarose gel and polyacrylamide gel electrophoresis. The ethidium bromide is combined with DNA and emits orange-red fluorescence under excitation by an ultraviolet light transmissive device, which can be photographed using a Polaroid negative film or a gel imaging processing system with a CCD imaging head. The fluorescence yield of the ethidium bromide-DNA complex is 20-30 times higher than that of the dye without DNA binding, so when the gel contains free ethidium bromide (0.5 ug/ml), it can be detected as little as 10 ng of DNA bands.
Usage: 1, Glue: 100ml agarose gel solution (concentration is generally 0.8%~2%) is melted in a microwave oven, cooled to about 60 °C (not hot), then add 5-10ul EB nucleic acid dye, Gently shake and pour the gel (to avoid air bubbles). After the gel is completely solidified, apply the electrophoresis. After electrophoresis, observe the photo under the violet light. (Note: due to the presence of the dye, the electrophoretic mobility of the linear DNA is about Reduce by 15%).
2. Bubbling: After agarose gel electrophoresis, the gel was immersed in running buffer containing EB (0.5 ug/ml) or deionized water, and stained at room temperature for 15-45 min (depending on gel thickness). Decolorization with deionized water or 1 mM MgSO4 solution for about 10-30 min at room temperature can reduce background fluorescence (optional).
Precautions:
1, this product is a strong mutagen, please pay attention to safety when using.
2, For your safety and health, please wear a lab coat and wear disposable gloves.
Related Products:
T1050 5×TBE buffer
T1060 50×TAE buffer
A1020 40% acrylamide (19:1)
D1010 6×DNA Lodding Buffe
M1060 D2000 DNA Ladder
G8140 GoldView Type I Nucleic Acid Stain (10000×)
G8142 GoldView ‖-type nucleic acid stain (5000×)
SY1020 SYBR Green I (10000×)
SY1040 SYBR Green (10000×)
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