EB Scavenger Instruction Item No.: E1027
Specification: 100T
Storage: sealed at room temperature and protected from light, valid for at least one year.
product manual:
This product achieves the purpose of removing EB contamination by destroying the molecular structure of EB. It is suitable for removing EB from solution and surface contamination, such as water, barium chloride solution, running buffer (TAE, TBE, MOPS, etc.), organic solvent (ethanol). , isopropanol,
Isoamyl alcohol, isobutanol, etc.) and the surface of a variety of contaminated objects (glass, stainless steel, plastic, flooring, UV filters, etc.). Easy to use and fast.
Steps:
One: remove EB from water-soluble solutions (such as water, Tris, MOPS, cesium chloride, etc.)
1. Dilute the solution with water to a concentration of EB below 1 mg/mL (if the EB concentration is below 1 mg/mL, proceed directly to the next step).
2. Add solution A and solution B to the solution according to the ratio of solution A: solution B: contaminated solution = 1:2:100 (there is a small amount of harmful gas generated in the initial stage of solution mixing, so the whole operation must be in the chemical fume hood Careful operation). Stir for five minutes.
3. Allow to stand at room temperature for 20 hours and neutralize with a saturated sodium bicarbonate solution to make the pH neutral.
4. Check the degree of removal and discard the waste.
Two: remove EB from cesium chloride saturated isopropanol
1. Dilute the solution with water to a concentration of EB below 1 mg/mL (if the EB concentration is below 1 mg/mL, proceed directly to the next step).
2. Add freshly prepared EB Erasol working solution in isopropyl alcohol-saturated isopropanol solution: EB Erasol working solution = 1:4 (see preparation method for seeing five).
3. Stir at room temperature for 20 hours. Neutralize with a saturated sodium bicarbonate solution to make the pH of the solution neutral.
4. Check the degree of removal and discard the waste.
Three: Elimination of EB in isoamyl alcohol and butanol
1. Dilute the solution with water to a concentration of EB below 1 mg/mL (if the EB concentration is below 1 mg/mL, proceed directly to the next step).
2. Add freshly prepared EB Erasol working solution according to the solution: EB Erasol working solution = 1:4 (see preparation method for seeing five)
3. The solution was divided into two phases and stirred at room temperature for 72 hours. Add the prepared activated carbon in a ratio of 2 g of activated carbon/100 mL of the mixture and stir for another 30 minutes. Filter to remove activated carbon. The liquid mixture was neutralized with saturated sodium bicarbonate to make the pH neutral.
4. Check the degree of removal and discard the waste.
Four: remove the EB from the surface of the object
1. Wipe the surface contamination of the object 5 times with a paper towel soaked in fresh EB Erasol working solution (see preparation method) for 5 times, each time replacing a new paper towel. Since the working fluid has a pH of 1.8, if the surface of the object is not resistant to acid (such as glass, stainless steel, floor, etc.), proceed directly to the second step. However, the general ultraviolet transmission filter can be directly treated with a working fluid.
2. Scrub the surface of the object with a paper towel soaked in water for 5 times, each time replacing a new tissue.
3. Check the cleaning effect with a UV lamp. If you do not see EB fluorescence, you can proceed to the next step. If there is still visible EB fluorescence, repeat the second step. (For the contamination that is not convenient for direct irradiation with UV lamps, the solution in the paper towel used can be extruded.
The intensity of fluorescence is relatively high when placed under an ultraviolet lamp, and the fluorescence generally becomes weaker.
4. Air dry the surface of the cleaned object. Soak the used paper towel in the EB Erasol working solution and let stand for at least one hour to degrade the EB.
5. Discard the paper towel. The working solution was neutralized with a self-prepared saturated sodium bicarbonate solution to make the pH neutral.
6. Check the degree of removal and discard the waste.
Five: Preparation of fresh EB Erasol working fluid
1. Estimate the amount of working fluid.
2. According to the solution A: solution B: water = 1:2:30, in the chemical fume hood, add water, solution A and solution B to the appropriate size container and stir for 10 minutes at room temperature (due to the preparation) A small amount of harmful gas is generated, so the entire operation must be handled carefully in a chemical fume hood.)
3. Immediately use freshly prepared working fluids as described above. Users need to wear gloves and wash them with tap water immediately after splashing on the skin.
Precautions:
1. Depending on the application, users may need to prepare saturated sodium bicarbonate and activated carbon.
2. This product is non-toxic, but the reagent itself and the operation may produce irritating and corrosive substances. It is necessary to wear gloves to operate in a ventilated place.
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10:1 Artichoke extract is one kind of Artichoke Extract which is our Herbal Product. It is a yellow brown powder and was producted by extracting,concentrating and drying;from the flower of Honeysuckle.The omponents are mainly polysaccharide and so on.
Artichoke
The involucre bracts and recepulus of artichoke bud can be eaten as vegetable, and the root can be used as medicine, which can enhance liver function and have diuretic effect.
Artichoke buds are nutrient-rich. Every 100 grams edible part contains water 86.5%, protein 2.8 grams, fat 0.2 grams, carbohydrate 9.9 grams, sustenant A160 international unit, vitamin B10.06 mg, vitamin C0.08 mg, vitamin 11 mg, calcium 51 mg, phosphorus 69 mg, iron 1.1 mg. The leaves contain artichoke, which has been shown to treat chronic hepatitis and lower cholesterol. The stems and leaves can be cooked after softening, and the taste is fresh.
Artichoke, also known as chrysanthemum thistle, cabbage thistle, French lily and lotus lily, is a perennial herb in the composite family.Origin of the sea coast, is from the thistle (C.cardunculus L.) evolved.Most cultivated in Italy.It was introduced to Shanghai from France in the 19th century.
China mainly in Shanghai, Zhejiang, Hunan, Yunnan and other places have a small amount of cultivation.The consumption and import of Artichoke (the name of the food in supermarkets is Artichoke) are increasing in developed countries such as the United States and Western Europe. Canned products are in short supply in the international market. In order to meet the needs of the international market, Taiwan Province of China has a large area of cultivation, and the products can be exported to earn foreign exchange.Artichokes are fed on flower buds and can be cooked after the petioles are softened and cultivated.
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