With the smog weather that has been frequently erupted in China in recent years, research on the impact of haze on human health has received more and more attention. In 2014 alone, several major scientific research projects were obtained by the Ministry of Science and Technology, the Ministry of Finance, etc. Strong support.
In China, due to the lack and deficiency of systematic epidemiological studies on the health effects of haze, the study of the health effects of respiratory smog exposure using experimental animals or human in vitro cultured cells has become toxicology, biomedical and environmental sciences. The most effective research methods in the field.
The study on the health effects of smog at the cellular level is not only the basis of all mechanistic studies, but also has the advantages of more targeted precision, high efficiency of experiments, and lower cost. At present, domestic cell exposure studies have started late, and experimental methods and levels are still relatively backward. There are great deficiencies in the design, evaluation methods and control of experimental variables of cell exposure experiments, which leads to great uncertainty in the research results.
As the highest level of scientific research in China, the Chinese Academy of Sciences, in 2014, pioneered the use of cultex gas-liquid interface cell exposure technology for smog health effects research in China, and proposed several innovative experimental protocols as a gas-liquid interface cell exposure technology. The invention and leader, Germany cultex has provided technical solutions for the research on the health effects of smog particles in China, and has been applied to a number of related major research projects. Among them, "the direct exposure of smog to the cultured cells in vitro at the gas-liquid interface" and "the establishment of a key particle model in smog" are of great significance for the study of the health effects of smog.
1. Direct exposure of smog to cultured cells in vitro at the gas-liquid interface
Figure 1 shows the direct exposure system of smog on the in vitro cultured cells at the gas-liquid interface.
This program has unparalleled technical advantages of traditional cell exposure:
1. Atmospheric particulate matter is exposed to cells at a gas-liquid interface in a real, real-time, direct manner;
2. The haze flow can be arbitrarily adjusted;
3, can be diluted to adjust the concentration of smog particles;
4. Particles are deposited vertically on the cell surface to minimize the effect of shear on cell and particulate matter deposition;
5. Simulate the respiratory barrier of lung exposure, reduce the large particles of agglomeration and the contact of condensed water droplets on the cell surface;
6. Using Cultex RFS radiation flow control technology, the experimental results have a high degree of experimental repeatability;
7, full-angle water bath, to ensure the stability of cell temperature environment in vitro, improve data repeatability; Â
Second, the establishment of key particle model in smog
At present, in the study of the health effects of smog particles, monodisperse particles are often used to replace smog with complex physicochemical properties, such as various PM2.5 (or PM10) standards or nano-scale smog ultrafine particles. However, the real haze particles have both primary particles and secondary particles formed by primary particles passing through atmospheric chemical reactions, and the secondary particles have greater toxic effects. There are certain gaps in the particle size, chemical composition and microbial composition of smog in different sources and regions. To this end, the study on the relationship between the concentration of different particulate matter and its mass concentration in smog, the study on the effect of microorganisms loaded in atmospheric particulate matter on the toxicity of smog particles, and the establishment of key particle model in smog are of great significance.
Based on its extensive experience in nanoparticle and whole cigarette smoke cell exposure, Cultex designed a multi-concentration gradient system with the same particle size distribution (Figure 2). Cultex Dust Generator (+HyP) was generated using fully automated microparticles dedicated to cell exposure. ), precise dispersion of particulates with a single dispersion of aerosol or mixed variables, and precise dilution to the desired concentration by a specially designed Cultex Aerosol Dilution. The use of this system can accurately control multiple variables on the same system, and understand the health effects or toxicity mechanisms of particulate matter at the cellular level, which greatly shortens the exploration time of particle model establishment.
 Â
Figure 2 shows the same particle size distribution multi-concentration gradient system established by the key particle model in haze Â
The system can be used for the health effects of carbon black particles, nanoparticles, carbon nanotubes, cigarette smoke, moon dust and various air-borne mixed particulate aerosols for in vitro cell exposure.
Pain Relief Patch for Breast
[Name] Medical Cold Patch
[Package Dimension] 10 round pieces
The pain relief patch is composed of three layers, namely, backing lining, middle gel and protective film. It is free from pharmacological, immunological or metabolic ingredients.
[Scope of Application] For cold physiotherapy, closed soft tissue only.
[Indications]
The patches give fast acting pain relief for breast hyperplasia, breast fibroids, mastitis, breast agglomera tion, swollen pain.
[How To Use a Patch]
Please follow the Schematic Diagram. One piece, one time.
The curing effect of each piece can last for 6-8 hours.
[Attention]
Do not apply the patch on the problematic skin, such as wounds, eczema, dermatitis,or in the eyes. People allergic to herbs and the pregnant are advised not to use the medication. If swelling or irritation occurs, please stop using and if any of these effects persist or worsen.notify your doctor or pharmacist promptly. Children using the patch must be supervised by adults.
[Storage Conditions]
Store below 30c in a dry place away from heat and direct sunlight.
Pain Relief Patch For Breast,Pain Relief Plaster For Breast,Relief For Breast Pain,Pad Relief Patch For Breast
Shandong XiJieYiTong International Trade Co.,Ltd. , https://www.xjpatches.com