How to use animal models to explore the mechanism of Nestin's function in neurodevelopment

Central nervous system development is an important event in the early development of animals. Nestin is an intermediate filament protein that is highly expressed in mammalian neural precursor cells and has been widely used as a marker for neural precursor cells. Therefore, the expression of nestin plays an important role in the analysis of the evolution of the nervous system, and can also be used as a rapid sensitive diagnostic indicator of neurological diseases and injuries. In adult tissues, nestin is expressed only in cells with partial differentiation potential, such as increased expression during skin and cardiovascular regeneration. In addition, nestin expression in a variety of tumor tissues increased, protein expression is positively correlated with the degree of malignancy of the tumor, so nestin is of great significance for the study of neurological diseases and tumor-related diseases.
A related study of Nestin knockout mice showed that in the heterozygous mice of the nestin+/- genotype, although the expression level of nestin was greatly reduced, it did not cause a visible phenotype. This indicates that the expression of nestin may not affect the development and survival of mice. However, homozygous mice, nestin-/- genotype mice have defects in neural tube development during embryonic development and die. This indicates that Nestin deficiency can cause a large number of apoptosis of neural tube cells, and the effect of apoptosis is not related to the cytoskeletal function of nestin.
Numerous studies have shown that Nestin has a special expression pattern: it is generally expressed in cells that are differentiated from immature cells, and its expression is decreased after cell differentiation and maturation until it is not expressed. Can Nestin overexpression maintain cell proliferation? Can overexpression during development affect the normal process of cell apoptosis? Nestin expression is also special: in general, nestin is expressed in the cytoplasm, but some cells are expressed on the nucleus. It suggests that nestin may interact with DNA or nuclear proteins to affect the expression of other genes by affecting the morphology or structure of chromosomes.

However, the early stage of Nestin complete knockout mouse embryos (E10.5) caused an in-depth study of the mechanism of Nestin's proliferation and differentiation in stem cells. Therefore, the Nestin gene knockout mouse model or the overexpressed mouse model can be used to inactivate/increase the Nestin gene at different stages of neurodevelopment, and to observe the loss/increased tissue organ formation at the global, cellular, and molecular levels. The relationship between development and function was studied to study the relationship between Nestin and neural stem/progenitor cell proliferation, differentiation, apoptosis and migration. This not only helps to elucidate the mechanism of Nestin's role in neurodevelopment, but also lays a solid foundation for exploring the role of Nestin in stem/progenitor cells of other tissues and organs (such as hair follicles, neovasculars, etc.).

Whether your research involves Nestin or other factors in the cellular process, Saiye Bio can customize the relevant knockout, knock-in or conditional knockout mouse models to help you make breakthroughs in your research.

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