The three principles that media experiments need to follow
First, the principle of preparation
The purpose is to clarify that a strong medium is formulated according to the nutritional requirements of different microorganisms. Autotrophic microorganisms can synthesize complex organic substances such as sugars, lipids, proteins, nucleic acids, and vitamins that are required from simple inorganic substances. Therefore, the medium for cultivating autotrophic microorganisms can be composed entirely of simple inorganic substances.
Second, the operation should be standardized
Bacterial culture, to master the temperature, humidity, time, environment and other factors, microbial limit test bacterial culture temperature is 30 ~ 35 ° C, this temperature is suitable for the growth of moderate temperature bacteria, some high temperature bacteria can also grow at this temperature. The control bacteria for drug contamination are mostly moderate temperature bacteria. Different culture times were selected according to different strains and test purposes. According to the different environmental requirements of bacteria, the commonly used culture methods are as follows
1. Aerobic culture method: This is the most commonly used culture method, suitable for the cultivation of aerobic bacteria and facultative anaerobic bacteria.
2. Anaerobic culture method: The cultivation of anaerobic bacteria must be carried out in an oxygen-free environment.
3. Aerobic culture method: Some bacteria are micro-aerobic bacteria in a micro-aerobic environment (5% 02, 10% CO2, 85% N2) grow well, micro-aerobic culture method commonly used mixed gas method, air bag method , double plate method, candle cylinder method.
Third, pay attention to economic savings
In the preparation of the culture medium, the raw materials should be used as cheap as possible and easily obtained. Especially in the fermentation industry, the medium is used in a large amount, and the low-cost raw materials are more economical. For example, in the industrial production of microbial single-cell protein, it is often used as a raw material for the use of molasses, soy product industrial waste liquid, etc., and a large number of agricultural by-products such as bran, rice bran, corn syrup, yeast extract, distiller's grains, Bean cakes, peanut cakes, etc. are commonly used fermentation industry raw materials.
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