Preparing samples
Sample pretreatment:
1. The ionic strength of the DNA sample is lower than that of the buffer.
2. The DNA sample is deproteinized
3. The sample load does not exceed 1.5ug
4. Understand the size of the DNA fragment to be recovered
5. 20 ul DNA sample (TE dissolved) + 5 ul marker/loading buffer (3%, 2%, 1.5%), mix and shake for centrifugation. 0.75% preset gel 20ul DNA sample + 5ul loading buffer, marker one lane directly 25ul.
Second programming
Under the Protocol Editor interface: 1. Select New Protocol 2. Select Preform Type 3. Set the size of the recovered clip 4. Specify the reference lane (internal/external) 5. By default, “Terminate after completionâ€.
Three optical correction (Pippin HT correction factor is 0.7)
Place the optical calibration plate with the black side facing down and place it in the lane card slot. Click “Calibrationâ€. Optical path correction must be performed before each experiment.
Four preparation prefabricated glue
1 Check prefabricated rubber
a Check the amount of buffer in the pre-formed gel, not enough.
b Check if the gel column breaks
c Check for bubbles
2 Prepare to load
a Remove the bubbles behind the elution hole
b Remove the seal and place the pre-formed glue in the instrument slot
c Aspirate 80 ul of buffer (3%, 2%, 1.5% of preset gel) from each buffer hole above; or pipette 150 ul of buffer (0.75% of preset gel).
d Empty the buffer in the elution well and add 30ul of fresh buffer
e Close the elution hole with tape
f Check the sample hole solution, when it is insufficient, fill up to 40ul
3 continuity check (test current. Continuity is related to temperature, so the buffer should be taken out of the refrigerator in advance for room temperature balance)
Five samples
Aspirate 30 ul of buffer, add 25 ul of sample, and close the lid.
Six running procedures
Seven recycling target segments
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