More than a hundred years ago, the elite in the lab began using pipettes to transfer liquids. The so-called pipette is a hollow glass tube with one to N scales on it. Insert the glass tube into the liquid and use the mouth on the other end of the tube (in the beginning, our elite can only use their precious mouth to dry the work) or wash the ear to suck the liquid into the tube, while above The scale tells us how much liquid is inside. After filling the amount we need, plug the end of the aspiration with your thumb, then place the tube in another container and let the liquid in the tube flow into the second container. This way we will be able to transfer the liquid!
However, with the progress of society, the elites found that the pipe was not easy to use, tired and slow, and it was not very clean. So the lazy thoughts accumulated in the brain, accumulated, and then broke out - so the pipette was born! That is to say, pipettes and pipettes have the same effect, the only difference is: First, the accuracy is higher (based on the accuracy of some new pipettes compared to the old pipettes) Sex is also very good); Second, higher efficiency (easy to operate); Third, the structure is more complicated (pipette is a tube, while pipettes are composed of dozens of parts); Fourth, the function is more Powerful (in addition to pipetting, many pipettes have many functions); five, and most importantly, the price is higher! (The price of high technology will definitely be higher.)
How pipettes work
For so-called precision instruments, most of the time you must always introduce its esoteric principles, but the principle of the pipette is very simple - the piston is moved up and down by the telescopic force of the spring to discharge or suck the liquid.
Generally speaking, pipettes are divided into two types, one is air displacement type; the other is external piston type, which is often used as a special pipette, and the application range is narrow. This type of pipette can be used. For the removal of samples with high viscosity.
The so-called air displacement type is to press the piston to press the air inside the lower end of the pipette, and then when the piston moves up, the air pressure inside the lower end of the pipette is smaller than the external air pressure, so that it can be under the action of the external air pressure. I sucked up the liquid. In short, the air goes out and the liquid comes in!
The so-called external piston type is actually the same as the syringe. When you see the working process of the syringe, you can probably understand the principle of the external piston. For those interested in the workings of pipettes, please refer to the previous article by Lebe, which has been specifically described in "Selection and use of pipettes - structure, principle and pipetting mode of pipettes".
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