Selection of RNA extraction reagents
RNA extraction is no stranger to researchers, but getting good results is not an easy task. In fact, the abundant RNA extraction reagents on the market can basically meet the needs of researchers. Why is the extracted RNA often easy to fail?
The main phenomenon of RNA extraction failure has three: the extraction of RNA degradation, low amounts of RNA extraction RNA extraction and low purity. How can we ensure the success rate of RNA extraction?
First, determine the availability of materials. Although existing RNA extraction reagents use RNase-inhibiting components, the extracted material still needs to be handled with care. The freshness of the extracted material is the key to obtaining intact RNA , but for various reasons it is not possible to extract RNA from fresh material immediately. Using Takara's sample protectant Sample Protector for RNA /DNA eliminates the use of liquid nitrogen or ultra-low temperature refrigerators. Inconvenience, it can also effectively solve short-term preservation and transportation problems of tissue and cell samples. In addition, if the samples collected in different periods are pre-stored in the reagent, the timing change of RNA expression can be terminated and fixed immediately, and the error between the experimental groups can be reduced.
Second, choosing the right extraction reagent is the most important step. Good extraction reagents are easy to operate and economical while ensuring success. Takara's RNAiso Plus has many success stories for animal tissues, simple plant materials, various microorganisms, and total RNA extraction from cultured cells. With the successful launch of the column extracting reagent TaKaRa MiniBEST Universal Total RNA Extraction Kit in July 2013, the product line of Takara RNA extraction was further enriched. The product uses a unique cell lysis system that eliminates the need for phenol chloroform extraction and is quick and easy. After tissue or cell lysis, the extraction can be completed in just 20 minutes.
It is difficult to extract plant-like tissue rich in polysaccharide polyphenols. Takara's carefully developed column extracting reagent TaKaRa MiniBEST Plant RNA Extraction Kit can easily solve this problem. TaKaRa MiniBEST Plant RNA Extraction Kit can efficiently extract high-purity Total RNA from various simple plant tissue materials (leaves, stems, seedlings, etc.), polysaccharide-rich polyphenol-containing plant tissue materials (fruits, seeds, etc.) and fungi. A wide range of applications. According to the standard protocol, the kit can efficiently extract RNA with a molecular weight greater than 200 nt, or follow the optional procedure to extract Total RNA containing Small RNA (<200 nt). The kit contains all the reagents needed for RNA extraction without the need to purchase additional reagents.
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